產品介紹

Confotec MR350, MR520, MR750

Compact Raman confocal microscopes Confotec™ MR350, Confotec™ MR520 and Confotec™ MR750 are intended for micro spectroscopic measurements with the high-end system capabilities.

產品說明

Compact Raman confocal microscopes  Confotec™ MR350, Confotec™ MR520 and Confotec™ MR750 
are intended for micro spectroscopic measurements with the high-end system capabilities.

All models have a rigid design that does not require adjustments and have both high sensitivity and high spatial resolution.

Spectral measurements are provided by a highly automated 350, 520 or 750 mm focal length Imaging monochromator-spectrograph with a motorized 4-position grating turret.

Performance capabilities, high reliability and compact design of confocal microscopes Confotec™ MR350, Confotec™ MR520, Confotec™ MR750 allow researchers to tackle different, crucial tasks in science and industry.

Semiconductors, liquid crystals, polymers, pharmaceutical and biological substances, single molecules and nanoparticles can be objects of research.

We offer 5 configurations of a Confotec™ Raman confocal microscope to realize optimal solutions for your needs.


Features

>  Modular design (the main Raman optical unit with a built-in laser is attached to the microscope, and the fiber coupled spectrograph can be arbitrarily located on a desktop or optical table)
>  Rigid design provides high thermal and temporal stability of spectral measurements, no pre-adjustment is needed
>  Nondestructive analysis, no special requirements for sample preparations
>  Two automatic switchable lasers with different wavelengths allow to measure  Raman spectra of  various samples without  influence of fluorescence background
>  Highly automated 350, 520 or 750 mm focal length Imaging monochromator-spectrograph with a motorized 4-position grating turret provides spectral measurements
>  Only some sample areas are located in focus due to limited confocal depth of field. A high-speed reflected unit allows you to quickly find a sample focus areas and use them for Raman measurements. This provides a significant gain in time and allows you to immediately use a high numerical aperture objective to record high spatial resolution Raman maps
>  High speed simultaneous Raman and Rayleigh sample imaging using PMT detectors and a XY galvano-mirror scanner
>  High speed and high spatial resolution ultra-wide panoramic imaging (Raman and Rayleigh scattering) using high speed PMTs, XY galvano-mirrors and an automated microscope stage
>  2D / 3D Raman imaging of a sample surface using XY galvano-mirror / Z-piezo scanners
>  Automatic spectral calibration


Configuration

The most complete configuration corresponds to the models Confotec™ MR350-SRMSp, Confotec™ MR520-SRMSp, Confotec™ MR750-SRMSp, comprising:

>  microscope with an automated stage
>  Raman module integrated in the optical microscope
>  two automatically switching lasers: a built-in laser (473 or 532 nm), and an external one (633 or 785 nm)
>  350, 520 or 750 mm focal length Imaging monochromator-spectrograph with an automated 4-position grating turret
>  digital color CCD camera for spectral measurements with Peltier cooling
>  high speed PMT unit for Raman imaging (3 sec / frame, 1001 x 1001 points) 
>  X-Y galvano-mirror scanner
>  Z-piezo scanner
>  reflected unit with a high speed detection system for Rayleigh imaging (3 sec / frame, 1001 x 1001 points) 

產品應用

Gemstone Identification
by Raman spectroscopy
 
This application note describes the use of confocal Raman 
spectroscopy for the identification of authentic materials
on the gem market.
more

 

 

     
Analysis of pharmaceutical
tabletsby Raman spectroscopy
 
Raman confocal microscopy is widely used in pharmaceutics. 
Such technique allows identify chemical compounds and 
molecular conformers in various drugs.
more
 

Polycrystalline silicon solar
cell inspection using confocal  
Raman microscopy

 
 
Energy from renewable sources, solar energy for example, 
is expected to play the important role in the near future. 
To convert the energy of sunlight directly into electricity, 
silicon solar cells (crystalline or polycrystalline) are widely used, 
because silicon technology is dominated in the market 
due to quickly reducing cost.
more
 
Characterization of diamond
coating tools by
photoluminescenceand Raman
spectroscopy
 
As diamond is a very hard material, diamond coating is
widely used in cutting or polishing tools.
To improve performance of diamond coating tools,
analysis techniques capable of test samples
with high spatial resolution are required. 
Raman/ photoluminescence spectroscopy is a good candidate
for such measurements due to ultra-high sensitivity
to the structural perfection of diamond.
more
 
The use of Raman confocal
methods for Depth Analysis
of Multilayer Polymer Films
 
    
 
Polymer multilayer films play an increasingly important role. 
Such films are used, for example, for food protection, 
as packing, insulating or coating material, etc. 
Confocal Raman microscopy is well suited tool for 
three-dimensional characterization of polymer films.
more

產品規格

Spatial resolution 

Laser Objective:
magnification and NA Lateral
Resolution (XY) Axial Resolution (Z)
473 nm 100x, NA = 0.95 390 nm 550 nm
532 nm 100x, NA = 0.95 440 nm 620 nm
633 nm 100x, NA = 0.95 520 nm 730 nm
785 nm 100x, NA = 0.95 650 nm 910 nm

 

Spectral parameters

Spectral range for Raman spectra detection: 60 сm-1 ~ 12000 сm-1 
(depends on the laser excitation wavelength)
Spectral resolution: 0.145 см-1 (Echelle grating, 75 l/mm , Confotec™ MR520)

 

Scanning

Model             Scanning mode
Confotec™ MR350-SRMSp

Confotec™ MR520-SRMSp

Confotec™ MR750-SRMSp
>  2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners
>  high speed 2D / 3D Raman imaging (3 sec / frame, 1001 x 1001 points) using a spectral PMT and XY galvano-mirror / Z-piezo scanners
>  high speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners
>  high speed and high spatial resolution panoramic imaging (Raman and Rayleigh scattering) using high speed PMTs, XY galvano-mirrors and an automated microscope stage
Confotec™ MR350-SRSp

Confotec™ MR520-SRSp

Confotec™ MR750-SRSp
>  2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners
>  high speed 2D / 3D Raman imaging (3 sec / frame, 1001 x 1001 points) using a spectral PMT and XY galvano-mirror / Z-piezo scanners
>  high speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners
Confotec™ MR350-SRM

Confotec™
MR520-SRM

Confotec™ MR750-SRM
>  2D / 3D Raman mapping of a sample  with full spectra detected  at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners
>  high speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners
>  high speed and high spatial resolution panoramic Rayleigh scattering imaging using a Reflected unit, XY galvano-mirrors and an automated microscope stage
Confotec™ MR350-SR

Confotec™ MR520-SR

Confotec™ MR750-SR
>  2D / 3D Raman mapping of a sample  with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners
>  
high speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners
Confotec™
MR350-S

Confotec™ 
MR520-S

Confotec™ 
MR750-S
>  2D / 3D Raman mapping of a sample  with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners
 

 

Microscope

Type, model Upright Nikon Ni-U
Objective lenses: 100х NA-0.95, 40х NA-0.75, 20х NA-0.50, etc.
Eyepieces: 10x, 12.5x, 15x
*Motorized stage:  
- travel range XY: X=114 mm, Y=75 mm
- position repeatability: < 0.5 μm
- metric accuracy: 0.06 μm / 1 mm
- minimal step: 0.02 μm
Manual focusing along Z:  
- travel range: 10 mm
- focusing mechanism sensitivity: 0.1 mm / turn
Z- scanner:  
- objective lens travel range: 80 μm
- minimal step: 50 nm
- repeatability: < 6 nm
High-resolution digital camera:  
With Peltier cooling  
- type: Digital color CCD camera 
- senso: 4/3", 2048 x 2048 pixels
- cooling: Peltier cooling (the temperature of 20 °C below the ambient temperature)
- pixel size: 7.4 x 7.4 μm
- ADC: 14 bit, 16 frames/sec
Without cooling:  
- type: Digital color CCD camera
- sensor: 1/2", 2048 x 1536 pixels
- pixel size: 3.2 x 3.2 μm
- ADC: 10 bit, 12 frames/sec
Laser light input: Three-position automatic turret

* for SRMSp and SRM configurations

 

Raman optical unit 

Optimized optics for the spectral range: VIS and NIR (400-1100 nm)
Laser radiation delivery: built-in  lasers   – direct input;
external lasers: 633 nm – fiber input, 
785 nm – direct input
Beam expander: magnification factor 2х – 10х
Edge filters unit: single position unit with two Edge filters,  manual change
Confocal pinhole: optical fiber (Ø 50 μm)
Laser beam attenuator: continuously Variable Neutral Density Filter (VND), 
optical density range of 0 - 3

 

Imaging monochromator-spectrograph  

  Confotec™ MR350 Confotec MR520 Confotec MR750
Monochromator-spectrograph model: MS3504i MS5204i MS7504i
Optical scheme: Czerny-Turner Czerny-Turner Czerny-Turner
Ports: 1 input, 2 output 1 input, 2 output 1 input, 2 output
Focal length (output): 350 520 750
F/number (entrance): 1 / 3.8 1 / 5.4 1 / 8.9
Horizontal magnification: 1.09 1.09 1.05
Vertical magnification: 1.49 1.29 1.125
Vertical spatial resolution (astigmatism) < 15 μm < 15 μm < 15 μm
Reciprocal linear dispersion: *2.37 nm/mm *1.50 nm/mm *1.02 nm/mm
Spectral resolution: *0.07 nm **0.007 (0.25 cm-1) nm *0.02 nm
Wavelength accuracy: *±0.125 nm *±0.05 nm *±0.05 nm
Repeatability: *± 0.03 nm *±0.008 nm *±0.006 nm
Grating mounting: Automated 4-grating turret Automated 4-grating turret Automated 4-grating turret
Grating wavelength repeatability: *±0.03 nm *±0.008 nm *±0.006 nm
Grating vertical image repeatability: ±50 μm ±50 μm ±50 μm

*  with 1200 l/mm grating and  15 µm slit at  546 nm
** with Echele grating (75 l / mm) and 15 µm slit at 546 nm, order 44

 

Imaging monochromator-spectrograph MS 3504i

   Laser wave-length, nm        
Grating, l/mm    300 600 1200 1800



Spectral range for Raman detection, cm-1   
473  0-12000    0-9400
532  0-9700    0-7000
633  0-6700    0-4000
 785  0-3600    0-1000
Maximal spectral range detected 
with CCD (2048x122 pixels, 12 μм pixel size), см-1
 473  7000 4210 2310 1550
 532  5700 3400 1840 1210
633 4280 2470 1300 820
785 2930 1650 830 480
Spectral resolution 
with CCD (12 μм pixel size, 14 μм entrance slit), см-1
473 12.850 6.440 3.185 2.050
532 10.165 5.090 2.505 1.590
633 7.190 3.595 1.745 1.070
785 4.675 2.330 1.100 0.620

 

 Imaging monochromator-spectrograph MS 5204i

  Laser wave-length, nm        
Grating, l/mm    300 600 1800 75/Echelle



Spectral range for Raman detection, cm-1  
473  0-12000   0-11000  0-12000
532  0-9700   0-8700  0-9700
633  0-6700   0-5800  0-6700
 785  0-3600   0-2700  0-3600
Maximal spectral range detected 
with CCD (2048x122 pixels, 12 μм pixel size), см-1
 473 6900 3500 1100 275
 532  5500 2760 850 255
633 3910 1950 570 210
785 2540 1260 330 160
Spectral resolution 
with CCD (12 μм pixel size, 14 μм entrance slit), см-1
473 6.150 3.080 0.975 0.250
532 4.865 2.430 0.755 0.230
633 3.440 1.715 0.505 0.190
785 2.235 1.110 0.295 0.145

 

Imaging monochromator-spectrograph MS 7504i

  Laser wave-length, nm        
Grating, l/mm    300 600 1200 1800



Spectral range for Raman detection, cm-1  
473  0-12000    0-9300
532  0-9700    0-6900
633  0-6700    0-3900
 785  0-3600    0-900
Maximal spectral range detected 
with CCD (2048x122 pixels, 12 μм pixel size), см-1
 473 3910 2130 1070 680
 532  3150 1690 840 520
633 2280 1200 580 340
785 1520 780 360 185
Spectral resolution 
with CCD (12 μм pixel size, 14 μм entrance slit), см-1
473 3.785 1.915 0.920 0.570
532 3.060 1.510 0.715 0.435
633 2.160 1.060 0.490 0.285
785 1.400 0.680 0.305 0.160

 

Digital spectral CCD camera

Sensor type: back-thinned CCD, 2048 x 122 pixels
Pixel size: 12 x 12 μm
Active area size: 24.576 x 1.464 mm (W x H)
Wavelength range: from 200 to 1100 nm
Cooling with temperature stabilization: two stage Peltier cooling, up to  - 45 °С (water cooling)
ADC: 16 bit
Sensitivity: 1 photon / 1 count ADC (at 650 nm)
Dynamic range: not less than 10 000
Synchronization: internal, external
Interface: Ethernet

 

PMT for spectral measurements

A Hamamatsu PMT unit (model  H7844 ) with the built-in one-stage  air cooled Peltier element is used for Ra-man spectra detection:

Spectral range: 185 - 900 nm
Photocathode light sensitivity: 450 μА/lm
Anode light sensitivity: >5000 А/lm
Anode dark current
(in 3 minutes after start cooling):
  
<0,05 nA
Max. cooling temperature (ΔТ): 20ºС

 

Fast scanning unit X, Y

Scanners: galvanometer scanners with X, Y mirrors
Scanning modes: raster high-speed and start-stop
Positioning accuracy: < 30 nm
Scanning area: 100 um х 100 um (with 100Х objective lens)
Scanning speed: 3 s / frame 1001 х 1001 pixels

 

Confocal Laser Microscope unit 

Pre-pinhole lens positioner: three-axis (X, Y, Z), manual control
Confocal pinhole: unregulated,  Ø 30 μm
Detector: Hamamatsu Photosensor module H6780-01

 

Lasers

532 nm built-in laser

Type: ОЕМ Diode-pumped solid-state laser (DPSS)
Wavelength: 532 nm
Output power: 22±2 mW
Output beam diameter: 1.1 ± 0.1 mm
Beam divergence: <0,6±0.1 mrad
Spectral line width: 0.00001 nm
Coherence length: >50 m
Transverse mode: TEMoo, M2 < 1.2 (1.05 typ.)
Polarization: >100:1, horizontal
Long term power stability: <2% / 8 h.
Warm-up time: <10 minutes

473 nm built-in laser

Type: Diode-pumped solid-state laser (DPSS)
Wavelength: 473 nm
Output power: 50 mW
Output beam diameter: 0.7 ± 0.05 mm
Beam divergence: <1.2 mrad
Spectral bandwidth: <30 mhz
Coherence length: >10 m
Transverse mode: TEMoo,  M2 < 1.1
Polarization: >100:1, vertical
Long term power stability: <3% / 8 h.
Beam point stability: <10 mrad/°C
Warm-up time: <10 minutes

633 nm external laser  

Type: Helium-neon laser
Wavelength: 632,8 nm
Output power: >10 mW
Output beam diameter: 0.65 mm
Beam divergence: <1,24 mrad
Spectral line width: 341 MHz
Mode composition: >90 % TEMoo
Polarization: 500:1
Long term power stability: <5% / 8 h.
Beam point stability: <0.03 mrad/°C
Warm-up time: <15 minutes

785 nm external laser  

Type: Diode-pumped solid-state, SLM laser
Wavelength: 785 ± 0.2 nm
Output power: 100 mW
Output beam diameter: 1.5 mm
Beam divergence: <1.0 mrad
Spectral bandwidth: <1 mhz=
Beam quality: M2 < 1.65
Polarization: vertical ±5°
Long term power stability: <1% / 8 h.
Warm-up time: <10 minutes

 

Models

  Confotec™ 
MR350/520/750-SRMSp
Confotec™ 
MR350/520/750-SRM

 
Confotec™ 
MR350/520/750-SRSp

 
Confotec™ 
MR350/520/750-SR

 
Confotec™ 
MR350/520/750-S

 
Mapping 3 D (X Y Z)  3 D (X Y Z) 3 D (X Y Z) 3 D (X Y Z) 3 D (X Y Z)
Scanning XY galvano + 
motorized stage,
Z piezo
XY galvano + 
motorized stage,
Z piezo
XY galvano,
Z piezo
XY galvano,
Z piezo
XY galvano,
Z piezo
Fast Scanning for Confocal 
Raman  imaging
Yes - Yes -  -
Fast Scanning for Confocal 
laser imaging
Yes Yes Yes Yes -
Data:  2D, 3D  2D, 3D 2D, 3D 2D, 3D 2D, 3D
Microscope upright
Color video CCD: Yes
Raman Optical Unit
Location: integration with an optical microscope
Optical alignment: no need
Rayleigh rejection filters: two edge filters, manually replacing
Imaging monochromator-spectrograph 
Focal length: 350/520/750 mm
Exit port: 2 1 2 1 1
Exit port selection: automated exit mirror
Grating: 4 4 4 4 4
Grating mounting: automated 4-grating turret
Light input: one-channel optical fiber
Digital spectral CCD camera
Number of pixels: 2048 x 122
Pixel size: 12 x 12 µм
Cooling with temperature stabilization:
two-stage Peltier cooling with temperature stabilization, up to   - 45°С (water cooling)
Excitation lasers (1 built-in and 1 external) 
Built-in laser  
Wavelength: 473 nm or 532 nm
Light input: direct
External laser  
Wavelength: 633 nm or 785 nm
Light input: 633 nm – fiber, 785 nm – direct
Laser beam diameter control Zoom beam expander, manual
Laser power control continuous VND (OD0-3), motorized 
Confocal pinhole  
Confocal Raman imaging: fiber Ø 50 μм
Confocal laser imaging: fixed Ø 30 μм fixed Ø 30 μм fixed Ø 30 μм fixed Ø 30 μм  
Reflected Unit Yes Yes Yes Yes -
Built-in calibration lamp           Yes
Spectral PMT Yes - Yes - -
Motorized microscope stage Yes Yes - - -