產品介紹
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Confotec MR150
It is a multifunction and compact confocal microscope. MR150 is the best Raman microscope in its class that deserves the maximum score for the price/quality criterion due to its spectral resolution, spectral calibration accuracy, universality, and ability to perform a variety of Raman measurements. Four configuration variants allow to choose the best system solution for your needs: MR150-SMR, MR150-SR, MR150-S and MR150.
產品說明
| > Fully integrated with a standard optical microscope > Rigid design, no preadjustment is needed > Compact spectrograph with a fixed position of Grating provides high thermal and temporal stability of spectral measurements > Nondestructive analysis, no special requirements for sample preparations > Two automatic switchable lasers with different wavelengths allow to measure the Raman spectra of the various samples without influence of fluorescence background > Two independent imaging spectrometers allow to optimize the spectral range for each of the two installed lasers or achieve an extremely high spectral resolution using Echelle grating in a spectrometer > Only some sample areas are located in focus due to limited confocal depth of field. A high-speed reflected unit allows you to quickly find a sample focus areas and to use them for Raman measurements. This provides a significant gain in time and allows you to immediately apply a high numerical aperture objective to record high spatial resolution Raman maps > High speed and high spatial resolution panoramic Rayleigh scattering imaging using a galvano-mirror scanner and an automated microscope stage > Automatic spectral calibration > The whole spectral range at once may be recorded, without the need for scanning and spectrometer tuning |
> Nondestructive analysis, no special requirements for sample preparations
> Two automatic switchable lasers with different wavelengths allow to measure the Raman spectra of the various samples without influence of fluorescence background
> Two independent imaging spectrometers allow to optimize the spectral range for each of the two installed lasers or achieve an extremely high spectral resolution using Echelle grating in a spectrometer
> Only some sample areas are located in focus due to limited confocal depth of field. A high-speed reflected unit allows you to quickly find a sample focus areas and to use them for Raman measurements. This provides a significant gain in time and allows you to immediately apply a high numerical aperture objective to record high spatial resolution Raman maps
> High speed and high spatial resolution panoramic Rayleigh scattering imaging using a galvano-mirror scanner and an automated microscope stage
> Automatic spectral calibration
> The whole spectral range at once may be recorded, without the need for scanning and spectrometer tuning
Configuration
The most complete configuration corresponds to the model MR150-SMR, consisting
> Microscope with an automated stage> Two manually switched lasers: one laser is integrated into the Raman system (473 nm or 532 nm), another one is external (633 nm or 785 nm)
> Two independent imaging spectrometers in one housing, each of which has a diffraction grating in the fixed position and a CCD detector cooled by the Peltier element
> X-Y galvanometer scanner
> Z- piezoscanner
> Reflected Unit for high speed imaging using Rayleigh scattering from a sample (3 sec / frame, 1001 x 1001 points)
產品規格
Spatial resolution
| Laser | Objective lens: magnification and numerical aperture NA |
Lateral resolution (XY) | Axial resolution (Z) |
| 473 nm | 100x, NA = 0.95 | 430 nm | 585 nm |
| 532 nm | 100x, NA = 0.95 | 525 nm | 705 nm |
| 633 nm | 100x, NA = 0.95 | 570 nm | 780 nm |
| 785 nm | 100x, NA = 0.95 | 995 nm | 1280 nm |
Spectral characteristics
| Spectral range for Raman spectra detection: | from 60 cm-1 to 11600 cm-1 (depends on the laser excitation wavelength) |
| Spectral resolution: | 0.9 cm-1 (Echelle, 75 l/mm) |
Scanning
| Model | Scanning modes |
|
Confotec |
> 2D / 3D Raman mapping of a sample with full spectra saving at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners > high speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners > high speed and high spatial resolution panoramic Rayleigh scattering imaging using a Reflected unit, XY galvano-mirrors and an automated mi-croscope stage |
| Confotec MR150-SR | > 2D / 3D Raman mapping of a sample with full spectra saving at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners> high speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners |
| Confotec MR150-S | > 2D / 3D Raman mapping of a sample with full spectra saving at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners |
| Confotec MR150 |
> One point Raman spectra measurement with a digital CCD camera |
Microscope
| Type, model: | Upright Nikon Ni-U |
| Objective lenses: | 100х NA-0.95, 40х NA-0.75, 20х NA-0.50 and other |
| Eyepieces: | 10x, 12.5x, 15x |
| * Motorized stage: | |
| - travel range XY: | X=114 mm, Y=75 mm |
| - position repeatability: | < 0.5 μm |
| - metrical accuracy: | 0.06 μm / 1 mm |
| - minimal step: | 0.02 μm |
| Manual focusing along Z: | |
| - travel range: | 10 mm |
| - focusing mechanism sensitivity: | 0.1 mm/turn |
| **Z-scanner: | |
| - objective lens travel range: | 80 μm |
| - minimal step: | 50 nm |
| - repeatability: | < 6 nm |
| High-resolution digital camera: | |
| With Peltier cooling | |
| - type: | Digital color CCD camera |
| - sensor: | 4/3", 2048 x 2048 pixels |
| - cooling: | Peltier cooling (the temperature of 20 °C below the ambient temperature) |
| - pixel size: | 7.4 x 7.4 μм |
| - ADC: | 14 bit, 16 frames / sec |
| Without cooling | |
| - type: | Digital color CCD camera |
| - sensor: | 1/2", 2048 x 1536 pixels |
| - pixel size: | 3.2 x 3.2 μм |
| - ADC: | 10 bit, 12 frames / sec |
| Laser light input: | Three-position automatic turret |
* For model Confotec MR150-SMR
** For models Confotec MR150-S, Confotec MR150-SR and Confotec MR150-SMR
Raman optical unit
| Optimized optics for the spectral range: | VIS and NIR (400-1100 nm) |
| Laser radiation delivery: | each of the built-in lasers – direct input; external lasers: 633 nm – fiber input, 785 nm – direct input |
| Beam expander: | Magnification factor 2х – 10х |
| Edge filters unit: | Monostatic unit with two Edge filters, manual change |
| Confocal pinhole: | Optical fiber Ø 50 μm |
| Laser beam attenuator: | continuously Variable Neutral Density Filter (VND), optical density range of 0 - 3 |
Imaging spectrograph S150
| Optical system: | |
| - optical design: | vertical-symmetric (two imaging spectrometers in one housing) |
| - focal length: | 158 mm |
| - focal plane: | 29 х 3 mm |
| - entrance slit: | optical fiber Ø 50 μм |
| - stray light level: | < 0,5 % |
| Astigmatism: | < 30 μм |
| Optical characteristics: | |
| Laser wavelength of 473nm (1200 l/mm grating, blaze 500nm) | |
| - wavelength range: | 473-615 nm (0-4880 cm-1) |
| - reciprocal linear dispersion: | ~4.96 nm/mm |
| - spectral resolution: | < 0.25 nm (8.35 cm-1) |
| - wavelength calibration accuracy: | < ±0.14 nm |
| Laser wavelength of 532nm (1200 l/mm grating, blaze 500nm) | |
| - wavelength range: | 532-672 nm (0-3920 cm-1) |
| - reciprocal linear dispersion: | ~4.9 nm/mm |
| - spectral resolution: | < 0.25 nm (6.73 cm-1) |
| - wavelength calibration accuracy: | < ±0.14 nm |
| Laser wavelength of 633nm (900 l/mm grating, blaze 600nm) | |
| - wavelength range: | 633-822 nm (0-3640 cm-1) |
| - reciprocal linear dispersion: | ~6.62 nm/mm |
| - spectral resolution: | < 0.34 nm (6.23 cm-1) |
| - wavelength calibration accuracy: | < ±0.19 nm |
| Laser wavelength of 785nm (600 l/mm grating, blaze of 750nm) | |
| - wavelength range: | 785-1073 nm (0-3430 cm-1) |
| - reciprocal linear dispersion: | ~10.1 nm/mm |
| - spectral resolution: | < 0.51 nm (5.82 cm-1) |
| - wavelength calibration accuracy: |
< ±0.29 nm |
|
For all lasers (Echelle 75 l/mm grating) |
 |
|
Registration system: |
Two channel, with independent channels |
| Photodetector type: | Back-Thinned CCD array (Hamamatsu, Japan), specially designed for use in spectrometers |
| Number of pixels: | 2048 х 64 |
| Pixel size: | 14 х 14 μм |
| Active area: | 28.672 х 0.896 mm (W x H) |
| Spectral range: | from 200 to 1100 nm |
| Cooling with temperature stabilization: | One-stage Peltier cooling to – 20° С (water cooling) |
| ADC: | 16 bit |
| Sensitivity: | 1 photon per 1 ADC count (at wavelength of 650 nm) |
| Dynamic range: | not less than 10 000 |
| Synchronization: | internal, external |
| Interface: | USB |
Fast scanning unit X, Y
| Scanners: | galvanometer scanners with X, Y mirrors |
| Scanning modes: | raster high-speed and start-stop |
| Positioning accuracy: | < 30 nm |
| Scanning area: | 100 μm х 100 μm (with 100Х objective lens) |
| Scanning speed: | 3 s/frame 1001 х 1001 pixels |
Confocal Laser Microscope unit
| Pre-pinhole lens positioner: | Three-axis (X, Y, Z), manual control |
| Confocal pinhole: | unregulated, Ø 30 μm |
| Detector: | Hamamatsu Photosensor module H6780-01 |
Lasers
532 nm built-in laser
| Type: | ОЕМ Diode-pumped solid-state laser (DPSS) |
| Wavelength: | 532 nm |
| Output power: | 22±2 mW |
| Output beam diameter: | 1.1 ± 0.1 mm |
| Beam divergence: | <0,6±0.1 mrad |
| Spectral line width: | 0.00001 nm |
| Coherence length: | >50 m |
| Transverse mode: | TEMoo, M2 < 1.2 (1.05 typ.) |
| Polarization: | > 100:1, horizontal |
| Long term power stability: | <2% / 8 h. |
| Warm-up time: | <10 minutes="" --10--=""> |
473 nm built-in laser
| Type: | ОЕМ Diode-pumped solid-state laser (DPSS) |
| Wavelength: | 473 nm |
| Output power: | 50 mW |
| Output beam diameter: | 0.7 ± 0.05 mm |
| Beam divergence: | <1.2 mrad="" --1="" 2--=""> |
| Spectral bandwidth: | <30 mhz="" --30--=""> |
| Coherence length: | >10 m |
| Transverse mode: | TEMoo, M2 < 1.1 |
| Polarization: | > 100:1, vertical |
| Long term power stability: | <3% / 8 h. |
| Beam point stability: | <10 mrad="" --10--=""> |
| Warm-up time: | <10 minutes="" --10--=""> |
633 nm built-in laser
| Type: | Helium-neon laser |
| Wavelength: | 632.8 nm |
| Output power: | >10 mW |
| Output beam diameter: | 0.65 mm |
| Beam divergence: | <1.24 mrad="" --1="" 24--=""> |
| Spectral bandwidth: | 341 MHz |
| Mode composition: | >90 % TEMoo |
| Polarization: | > 500:1 |
| Long term power stability: | <5% / 8 h. |
| Beam point stability: | <0.03 mrad="" --0="" 03--=""> |
| Warm-up time: | <15 minutes="" --15--=""> |
785 nm built-in laser
| Type: | Diode-pumped solid-state laser (DPSS |
| Wavelength: | 785 ± 0.2 nm |
| Output power: | 100 mW |
| Output beam diameter: | 1.5 mm |
| Beam divergence: | <1.0 mrad="" --1="" 0--=""> |
| Spectral bandwidth: | <1 mhz="" --1--=""> |
| Beam quality: | M2 < 1.65 |
| Polarization: | Vertical ±5° |
| Long term power stability: | <1% / 8 h. |
| Warm-up time: | <10 minutes="" --10--=""> |
Models
| Confotec MR150-SMR | Confotec MR150-SR | Confotec MR150-S | Confotec MR150 | |
| Mapping | 3D (X Y Z) | 3D (X Y Z) | 3D (X Y Z) | - |
| Scanning: | XY galvano + motorized stage, Z piezo |
XY galvano, Z piezo |
XY galvano, Z piezo |
- |
| Fast Scanning for Confocal laser imaging |
Yes | Yes | - | - |
| Data: | 2D, 3D | 2D, 3D | 2D, 3D | - |
| Microscope | Upright | |||
| Color video CCD: | Yes | |||
| Raman optical unit | ||||
| Location: | integration with an optical microscope | |||
| Optical alignment: | no need | |||
| Rayleigh rejection filters | two edge filters, manually replacing | |||
| Imaging Spectrograph | ||||
| Location: | integration with an optical microscope | |||
| Optical design: | two independent imaging spectrometers in one housing with a built-in two-channel registration system | |||
| Focal length: | 150 mm | |||
| Exit ports: | 2 | |||
| Grating: | 2 | |||
| Light input: | two-channel optical fiber | |||
| Channel switching: | automated | |||
| Registration system: | two channel, with independent channels | |||
| Detector (each channel): | ||||
| Number of pixels: | 2048x64 | |||
| Pixel size: | 14x14 μm | |||
| Sensor cooling: | one-stage Peltier cooling with temperature stabilization | |||
| Cooling method: | liquid cooling | |||
| Excitation laser | ||||
| Built-in laser | ||||
| Wavelength: | 473 nm или 532 nm | |||
| Light input: | direct | |||
| External laser | ||||
| Wavelength: | 633 nm или 785 nm | |||
| Light input: | 633 nm – fiber, 785 n – direct | |||
| Laser beam diameter control | zoom beam expander, manual | |||
| Laser power control | continuous VND (OD0-3), motorized | |||