產品介紹
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Confotec MR350, MR520, MR750
Compact Raman confocal microscopes Confotec™ MR350, Confotec™ MR520 and Confotec™ MR750 are intended for micro spectroscopic measurements with the high-end system capabilities.
產品說明
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Compact Raman confocal microscopes Confotec™ MR350, Confotec™ MR520 and Confotec™ MR750 All models have a rigid design that does not require adjustments and have both high sensitivity and high spatial resolution. Spectral measurements are provided by a highly automated 350, 520 or 750 mm focal length Imaging monochromator-spectrograph with a motorized 4-position grating turret. Performance capabilities, high reliability and compact design of confocal microscopes Confotec™ MR350, Confotec™ MR520, Confotec™ MR750 allow researchers to tackle different, crucial tasks in science and industry. Semiconductors, liquid crystals, polymers, pharmaceutical and biological substances, single molecules and nanoparticles can be objects of research. We offer 5 configurations of a Confotec™ Raman confocal microscope to realize optimal solutions for your needs. |
Features
> Rigid design provides high thermal and temporal stability of spectral measurements, no pre-adjustment is needed
> Nondestructive analysis, no special requirements for sample preparations
> Two automatic switchable lasers with different wavelengths allow to measure Raman spectra of various samples without influence of fluorescence background
> Highly automated 350, 520 or 750 mm focal length Imaging monochromator-spectrograph with a motorized 4-position grating turret provides spectral measurements
> Only some sample areas are located in focus due to limited confocal depth of field. A high-speed reflected unit allows you to quickly find a sample focus areas and use them for Raman measurements. This provides a significant gain in time and allows you to immediately use a high numerical aperture objective to record high spatial resolution Raman maps
> High speed simultaneous Raman and Rayleigh sample imaging using PMT detectors and a XY galvano-mirror scanner
> High speed and high spatial resolution ultra-wide panoramic imaging (Raman and Rayleigh scattering) using high speed PMTs, XY galvano-mirrors and an automated microscope stage
> 2D / 3D Raman imaging of a sample surface using XY galvano-mirror / Z-piezo scanners
> Automatic spectral calibration
Configuration
The most complete configuration corresponds to the models Confotec™ MR350-SRMSp, Confotec™ MR520-SRMSp, Confotec™ MR750-SRMSp, comprising:
> microscope with an automated stage> Raman module integrated in the optical microscope
> two automatically switching lasers: a built-in laser (473 or 532 nm), and an external one (633 or 785 nm)
> 350, 520 or 750 mm focal length Imaging monochromator-spectrograph with an automated 4-position grating turret
> digital color CCD camera for spectral measurements with Peltier cooling
> high speed PMT unit for Raman imaging (3 sec / frame, 1001 x 1001 points)
> X-Y galvano-mirror scanner
> Z-piezo scanner
> reflected unit with a high speed detection system for Rayleigh imaging (3 sec / frame, 1001 x 1001 points)
產品應用
| Gemstone Identification by Raman spectroscopy |
This application note describes the use of confocal Raman
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| Analysis of pharmaceutical tabletsby Raman spectroscopy |
Raman confocal microscopy is widely used in pharmaceutics.
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Polycrystalline silicon solar cell inspection using confocal Raman microscopy |
Energy from renewable sources, solar energy for example,
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| Characterization of diamond coating tools by photoluminescenceand Raman spectroscopy |
As diamond is a very hard material, diamond coating is
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| The use of Raman confocal methods for Depth Analysis of Multilayer Polymer Films |
Polymer multilayer films play an increasingly important role.
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產品規格
Spatial resolution
| Laser | Objective: magnification and NA Lateral |
Resolution (XY) Axial | Resolution (Z) |
| 473 nm | 100x, NA = 0.95 | 390 nm | 550 nm |
| 532 nm | 100x, NA = 0.95 | 440 nm | 620 nm |
| 633 nm | 100x, NA = 0.95 | 520 nm | 730 nm |
| 785 nm | 100x, NA = 0.95 | 650 nm | 910 nm |
Spectral parameters
| Spectral range for Raman spectra detection: | 60 сm-1 ～ 12000 сm-1 (depends on the laser excitation wavelength) |
| Spectral resolution: | 0.145 см-1 (Echelle grating, 75 l/mm , Confotec™ MR520) |
Scanning
| Model | Scanning mode |
| Confotec™ MR350-SRMSp Confotec™ MR520-SRMSp Confotec™ MR750-SRMSp |
> 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners > high speed 2D / 3D Raman imaging (3 sec / frame, 1001 x 1001 points) using a spectral PMT and XY galvano-mirror / Z-piezo scanners > high speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners > high speed and high spatial resolution panoramic imaging (Raman and Rayleigh scattering) using high speed PMTs, XY galvano-mirrors and an automated microscope stage |
| Confotec™ MR350-SRSp Confotec™ MR520-SRSp Confotec™ MR750-SRSp |
> 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners > high speed 2D / 3D Raman imaging (3 sec / frame, 1001 x 1001 points) using a spectral PMT and XY galvano-mirror / Z-piezo scanners > high speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners |
| Confotec™ MR350-SRM Confotec™ MR520-SRM Confotec™ MR750-SRM |
> 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners > high speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners > high speed and high spatial resolution panoramic Rayleigh scattering imaging using a Reflected unit, XY galvano-mirrors and an automated microscope stage |
| Confotec™ MR350-SR Confotec™ MR520-SR Confotec™ MR750-SR |
> 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners > high speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners |
| Confotec™ MR350-S Confotec™ MR520-S Confotec™ MR750-S |
> 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners |
Microscope
| Type, model | Upright Nikon Ni-U |
| Objective lenses: | 100х NA-0.95, 40х NA-0.75, 20х NA-0.50, etc. |
| Eyepieces: | 10x, 12.5x, 15x |
| *Motorized stage: | |
| - travel range XY: | X=114 mm, Y=75 mm |
| - position repeatability: | < 0.5 μm |
| - metric accuracy: | 0.06 μm / 1 mm |
| - minimal step: | 0.02 μm |
| Manual focusing along Z: | |
| - travel range: | 10 mm |
| - focusing mechanism sensitivity: | 0.1 mm / turn |
| Z- scanner: | |
| - objective lens travel range: | 80 μm |
| - minimal step: | 50 nm |
| - repeatability: | < 6 nm |
| High-resolution digital camera: | |
| With Peltier cooling | |
| - type: | Digital color CCD camera |
| - senso: | 4/3", 2048 x 2048 pixels |
| - cooling: | Peltier cooling (the temperature of 20 °C below the ambient temperature) |
| - pixel size: | 7.4 x 7.4 μm |
| - ADC: | 14 bit, 16 frames/sec |
| Without cooling: | |
| - type: | Digital color CCD camera |
| - sensor: | 1/2", 2048 x 1536 pixels |
| - pixel size: | 3.2 x 3.2 μm |
| - ADC: | 10 bit, 12 frames/sec |
| Laser light input: | Three-position automatic turret |
* for SRMSp and SRM configurations
Raman optical unit
| Optimized optics for the spectral range: | VIS and NIR (400-1100 nm) |
| Laser radiation delivery: | built-in lasers – direct input; external lasers: 633 nm – fiber input, 785 nm – direct input |
| Beam expander: | magnification factor 2х – 10х |
| Edge filters unit: | single position unit with two Edge filters, manual change |
| Confocal pinhole: | optical fiber (Ø 50 μm) |
| Laser beam attenuator: | continuously Variable Neutral Density Filter (VND), optical density range of 0 - 3 |
Imaging monochromator-spectrograph
| Confotec™ MR350 | Confotec™ MR520 | Confotec™ MR750 | |
| Monochromator-spectrograph model: | MS3504i | MS5204i | MS7504i |
| Optical scheme: | Czerny-Turner | Czerny-Turner | Czerny-Turner |
| Ports: | 1 input, 2 output | 1 input, 2 output | 1 input, 2 output |
| Focal length (output): | 350 | 520 | 750 |
| F/number (entrance): | 1 / 3.8 | 1 / 5.4 | 1 / 8.9 |
| Horizontal magnification: | 1.09 | 1.09 | 1.05 |
| Vertical magnification: | 1.49 | 1.29 | 1.125 |
| Vertical spatial resolution (astigmatism) | < 15 μm | < 15 μm | < 15 μm |
| Reciprocal linear dispersion: | *2.37 nm/mm | *1.50 nm/mm | *1.02 nm/mm |
| Spectral resolution: | *0.07 nm | **0.007 (0.25 cm-1) nm | *0.02 nm |
| Wavelength accuracy: | *±0.125 nm | *±0.05 nm | *±0.05 nm |
| Repeatability: | *± 0.03 nm | *±0.008 nm | *±0.006 nm |
| Grating mounting: | Automated 4-grating turret | Automated 4-grating turret | Automated 4-grating turret |
| Grating wavelength repeatability: | *±0.03 nm | *±0.008 nm | *±0.006 nm |
| Grating vertical image repeatability: | ±50 μm | ±50 μm | ±50 μm |
* with 1200 l/mm grating and 15 µm slit at 546 nm
** with Echele grating (75 l / mm) and 15 µm slit at 546 nm, order 44
Imaging monochromator-spectrograph MS 3504i
| Laser wave-length, nm | |||||
| Grating, l/mm | 300 | 600 | 1200 | 1800 | |
Spectral range for Raman detection, cm-1 |
473 | 0-12000 | 0-9400 | ||
| 532 | 0-9700 | 0-7000 | |||
| 633 | 0-6700 | 0-4000 | |||
| 785 | 0-3600 | 0-1000 | |||
| Maximal spectral range detected with CCD (2048x122 pixels, 12 μм pixel size), см-1 |
473 | 7000 | 4210 | 2310 | 1550 |
| 532 | 5700 | 3400 | 1840 | 1210 | |
| 633 | 4280 | 2470 | 1300 | 820 | |
| 785 | 2930 | 1650 | 830 | 480 | |
| Spectral resolution with CCD (12 μм pixel size, 14 μм entrance slit), см-1 |
473 | 12.850 | 6.440 | 3.185 | 2.050 |
| 532 | 10.165 | 5.090 | 2.505 | 1.590 | |
| 633 | 7.190 | 3.595 | 1.745 | 1.070 | |
| 785 | 4.675 | 2.330 | 1.100 | 0.620 | |
Imaging monochromator-spectrograph MS 5204i
| Laser wave-length, nm | |||||
| Grating, l/mm | 300 | 600 | 1800 | 75/Echelle | |
Spectral range for Raman detection, cm-1 |
473 | 0-12000 | 0-11000 | 0-12000 | |
| 532 | 0-9700 | 0-8700 | 0-9700 | ||
| 633 | 0-6700 | 0-5800 | 0-6700 | ||
| 785 | 0-3600 | 0-2700 | 0-3600 | ||
| Maximal spectral range detected with CCD (2048x122 pixels, 12 μм pixel size), см-1 |
473 | 6900 | 3500 | 1100 | 275 |
| 532 | 5500 | 2760 | 850 | 255 | |
| 633 | 3910 | 1950 | 570 | 210 | |
| 785 | 2540 | 1260 | 330 | 160 | |
| Spectral resolution with CCD (12 μм pixel size, 14 μм entrance slit), см-1 |
473 | 6.150 | 3.080 | 0.975 | 0.250 |
| 532 | 4.865 | 2.430 | 0.755 | 0.230 | |
| 633 | 3.440 | 1.715 | 0.505 | 0.190 | |
| 785 | 2.235 | 1.110 | 0.295 | 0.145 | |
Imaging monochromator-spectrograph MS 7504i
| Laser wave-length, nm | |||||
| Grating, l/mm | 300 | 600 | 1200 | 1800 | |
Spectral range for Raman detection, cm-1 |
473 | 0-12000 | 0-9300 | ||
| 532 | 0-9700 | 0-6900 | |||
| 633 | 0-6700 | 0-3900 | |||
| 785 | 0-3600 | 0-900 | |||
| Maximal spectral range detected with CCD (2048x122 pixels, 12 μм pixel size), см-1 |
473 | 3910 | 2130 | 1070 | 680 |
| 532 | 3150 | 1690 | 840 | 520 | |
| 633 | 2280 | 1200 | 580 | 340 | |
| 785 | 1520 | 780 | 360 | 185 | |
| Spectral resolution with CCD (12 μм pixel size, 14 μм entrance slit), см-1 |
473 | 3.785 | 1.915 | 0.920 | 0.570 |
| 532 | 3.060 | 1.510 | 0.715 | 0.435 | |
| 633 | 2.160 | 1.060 | 0.490 | 0.285 | |
| 785 | 1.400 | 0.680 | 0.305 | 0.160 | |
Digital spectral CCD camera
| Sensor type: | back-thinned CCD, 2048 x 122 pixels |
| Pixel size: | 12 x 12 μm |
| Active area size: | 24.576 x 1.464 mm (W x H) |
| Wavelength range: | from 200 to 1100 nm |
| Cooling with temperature stabilization: | two stage Peltier cooling, up to - 45 °С (water cooling) |
| ADC: | 16 bit |
| Sensitivity: | 1 photon / 1 count ADC (at 650 nm) |
| Dynamic range: | not less than 10 000 |
| Synchronization: | internal, external |
| Interface: | Ethernet |
PMT for spectral measurements
A Hamamatsu PMT unit (model H7844 ) with the built-in one-stage air cooled Peltier element is used for Ra-man spectra detection:
| Spectral range: | 185 - 900 nm |
| Photocathode light sensitivity: | 450 μА/lm |
| Anode light sensitivity: | >5000 А/lm |
| Anode dark current (in 3 minutes after start cooling): |
<0,05 nA |
| Max. cooling temperature (ΔТ): | 20ºС |
Fast scanning unit X, Y
| Scanners: | galvanometer scanners with X, Y mirrors |
| Scanning modes: | raster high-speed and start-stop |
| Positioning accuracy: | < 30 nm |
| Scanning area: | 100 um х 100 um (with 100Х objective lens) |
| Scanning speed: | 3 s / frame 1001 х 1001 pixels |
Confocal Laser Microscope unit
| Pre-pinhole lens positioner: | three-axis (X, Y, Z), manual control |
| Confocal pinhole: | unregulated, Ø 30 μm |
| Detector: | Hamamatsu Photosensor module H6780-01 |
Lasers
532 nm built-in laser
| Type: | ОЕМ Diode-pumped solid-state laser (DPSS) |
| Wavelength: | 532 nm |
| Output power: | 22±2 mW |
| Output beam diameter: | 1.1 ± 0.1 mm |
| Beam divergence: | <0,6±0.1 mrad |
| Spectral line width: | 0.00001 nm |
| Coherence length: | >50 m |
| Transverse mode: | TEMoo, M2 < 1.2 (1.05 typ.) |
| Polarization: | >100:1, horizontal |
| Long term power stability: | <2% / 8 h. |
| Warm-up time: | <10 minutes |
473 nm built-in laser
| Type: | Diode-pumped solid-state laser (DPSS) |
| Wavelength: | 473 nm |
| Output power: | 50 mW |
| Output beam diameter: | 0.7 ± 0.05 mm |
| Beam divergence: | <1.2 mrad |
| Spectral bandwidth: | <30 mhz |
| Coherence length: | >10 m |
| Transverse mode: | TEMoo, M2 < 1.1 |
| Polarization: | >100:1, vertical |
| Long term power stability: | <3% / 8 h. |
| Beam point stability: | <10 mrad/°C |
| Warm-up time: | <10 minutes |
633 nm external laser
| Type: | Helium-neon laser |
| Wavelength: | 632,8 nm |
| Output power: | >10 mW |
| Output beam diameter: | 0.65 mm |
| Beam divergence: | <1,24 mrad |
| Spectral line width: | 341 MHz |
| Mode composition: | >90 % TEMoo |
| Polarization: | 500:1 |
| Long term power stability: | <5% / 8 h. |
| Beam point stability: | <0.03 mrad/°C |
| Warm-up time: | <15 minutes |
785 nm external laser
| Type: | Diode-pumped solid-state, SLM laser |
| Wavelength: | 785 ± 0.2 nm |
| Output power: | 100 mW |
| Output beam diameter: | 1.5 mm |
| Beam divergence: | <1.0 mrad |
| Spectral bandwidth: | <1 mhz= |
| Beam quality: | M2 < 1.65 |
| Polarization: | vertical ±5° |
| Long term power stability: | <1% / 8 h. |
| Warm-up time: | <10 minutes |
Models
| Confotec™ MR350/520/750-SRMSp |
Confotec™ MR350/520/750-SRM |
Confotec™ MR350/520/750-SRSp |
Confotec™ MR350/520/750-SR |
Confotec™ MR350/520/750-S |
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| Mapping | 3 D (X Y Z) | 3 D (X Y Z) | 3 D (X Y Z) | 3 D (X Y Z) | 3 D (X Y Z) |
| Scanning | XY galvano + motorized stage, Z piezo |
XY galvano + motorized stage, Z piezo |
XY galvano, Z piezo |
XY galvano, Z piezo |
XY galvano, Z piezo |
| Fast Scanning for Confocal Raman imaging |
Yes | - | Yes | - | - |
| Fast Scanning for Confocal laser imaging |
Yes | Yes | Yes | Yes | - |
| Data: | 2D, 3D | 2D, 3D | 2D, 3D | 2D, 3D | 2D, 3D |
| Microscope | upright | ||||
| Color video CCD: | Yes | ||||
| Raman Optical Unit | |||||
| Location: | integration with an optical microscope | ||||
| Optical alignment: | no need | ||||
| Rayleigh rejection filters: | two edge filters, manually replacing | ||||
| Imaging monochromator-spectrograph | |||||
| Focal length: | 350/520/750 mm | ||||
| Exit port: | 2 | 1 | 2 | 1 | 1 |
| Exit port selection: | automated exit mirror | ||||
| Grating: | 4 | 4 | 4 | 4 | 4 |
| Grating mounting: | automated 4-grating turret | ||||
| Light input: | one-channel optical fiber | ||||
| Digital spectral CCD camera | |||||
| Number of pixels: | 2048 x 122 | ||||
| Pixel size: | 12 x 12 µм | ||||
| Cooling with temperature stabilization: | two-stage Peltier cooling with temperature stabilization, up to - 45°С (water cooling) |
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| Excitation lasers (1 built-in and 1 external) | |||||
| Built-in laser | |||||
| Wavelength: | 473 nm or 532 nm | ||||
| Light input: | direct | ||||
| External laser | |||||
| Wavelength: | 633 nm or 785 nm | ||||
| Light input: | 633 nm – fiber, 785 nm – direct | ||||
| Laser beam diameter control | Zoom beam expander, manual | ||||
| Laser power control | continuous VND (OD0-3), motorized | ||||
| Confocal pinhole | |||||
| Confocal Raman imaging: | fiber Ø 50 μм | ||||
| Confocal laser imaging: | fixed Ø 30 μм | fixed Ø 30 μм | fixed Ø 30 μм | fixed Ø 30 μм | |
| Reflected Unit | Yes | Yes | Yes | Yes | - |
| Built-in calibration lamp | Yes | ||||
| Spectral PMT | Yes | - | Yes | - | - |
| Motorized microscope stage | Yes | Yes | - | - | - |